Determination and pharmacokinetics of gastrodin in human plasma by HPLC coupled with photodiode array detector

Abstract

In present study, an HPLC method coupled with photodiode array detector (HPLC-PDA) was established for determination and pharmacokinetics of gastrodin (GAS) in human plasma after an oral administration of GAS capsule. In the method, ethanol and dichloromethane were respectively used for deproteinization and purification during the sample preparation procedure. Separation of GAS was achieved on an AichromBond-AQ C18 column (5 microm, 150 mm x 4.6 mm) with the mobile phase of methanol-0.1% phosphoric acid solution (2:98, v/v) at a flow rate of 0.8 ml/min. The wavelength was set at 220 nm and the injection volume was 20 microl. Under the conditions, the calibration curve was linear within the concentration range of 50-4000 ng/ml with the correlation coefficient (r) of 0.99554 (weight=1/X(2)) and the lower limit of quantification (LLOQ) was 50 ng/ml. The inter- and intra-day precisions were less than 11% and the accuracies (%) were within the range of 95.55-103.78%. The extraction recoveries were over 65% with RSDs less than 5.50%. The GAS was proved to be stable under tested conditions. Thus, the method was valid enough to be applied for pharmacokinetic study of GAS in human plasma. The pharmacokinetic parameters of GAS in human plasma after an oral administration of 200 mg GAS capsule were described as: C(max), 1484.55+/-285.05 ng/ml; T(max), 0.81+/-0.16 h; t(1/2alpha), 3.78+/-2.33 h; t(1/2beta), 6.06+/-3.20 h; t(1/2Ka), 0.18+/-0.53 h; K(12), 0.18+/-0.41/h; K(21), 0.20+/-0.16/h; K(10), 4.11+/-15.81/h; V1/F, 180.35+/-89.44 L; CL/F, 62.50+/-140.03 l/h; AUC(0-->t), 5619.41+/-1972.88 (ng/ml) h; and AUC(0-->infinity), 7210.26+/-3472.74 (ng/ml) h, respectively. These will be useful for the clinical application of GAS.