Evolutionary adaptation of recombinant shochu yeast for improved xylose utilization

Akinori Matsushika; Emiko Oguri; Shigeki Sawayama

doi:10.1016/j.jbiosc.2010.01.002

Evolutionary adaptation of recombinant shochu yeast for improved xylose utilization

J Biosci Bioeng. 2010 Jul;110(1):102-5. doi: 10.1016/j.jbiosc.2010.01.002. Epub 2010 Jan 27.

Authors

Akinori Matsushika¹, Emiko Oguri, Shigeki Sawayama

Affiliation

¹ Biomass Technology Research Center, National Institute of Advanced Industrial Science and Technology, 2-2-2 Hirosuehiro, Kure, Hiroshima 737-0197, Japan. a-matsushika@aist.go.jp

PMID: 20541125
DOI: 10.1016/j.jbiosc.2010.01.002

Abstract

We examined the evolutionary adaptation of recombinant shochu yeast by serial anaerobic cultivation in xylose-based minimal medium. Compared with the parental strain, the adapted strain MA-S4-M1 (M1) markedly improved the growth on xylose and the anaerobic xylose consumption rate. M1 gained improved xylose utilization properties by optimizing the metabolic pathway enzymes and enhancing the uptake of xylose.

2010. Published by Elsevier B.V.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adaptation, Physiological / genetics
Adaptation, Physiological / physiology*
Anaerobiosis
Biological Evolution*
Saccharomyces cerevisiae / enzymology
Saccharomyces cerevisiae / genetics*
Saccharomyces cerevisiae / growth & development
Saccharomyces cerevisiae / metabolism
Time Factors
Xylose / metabolism*

Substances

Xylose