The Foss NIRSystem 6500 is one of the most commonly used laboratory instruments in agriculture and in particular in feed. New technological developments include micro-electro mechanical system (MEMS) technology, used in miniature handheld instruments such as the Polychromix Phazir spectrometer that are increasingly required for on-site analysis. The objective of this study was to assess the potential of a calibration transfer from the Foss NIRSystem 6500 to the Polychromix Phazir. The results show that good calibration models were obtained for various feed properties (fat, fiber, protein, and starch) developed on a Foss NIRSystem 6500, based on a spectral database of 9164 samples transferred to a Polychromix Phazir handheld spectrometer.