Immobilization of trypsin on water-soluble dendrimer-modified carbon nanotubes for on-plate proteolysis combined with MALDI-MS analysis

Mol Biosyst. 2010 Aug;6(8):1447-9. doi: 10.1039/c004911e. Epub 2010 Jun 9.

Abstract

A novel on-plate digestion method combined with MALDI-MS analysis is reported, using trypsin-linked dendrimer-modified carbon nanotubes (dCNTs) as the enzyme immobilization probe. Excellent digestion performance was achieved in a short time without any complicated reduction and alkylation procedures.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Combinatorial Chemistry Techniques / instrumentation
  • Combinatorial Chemistry Techniques / methods
  • Dendrimers / chemistry
  • Dendrimers / metabolism*
  • Enzymes, Immobilized* / chemical synthesis
  • Enzymes, Immobilized* / chemistry
  • Enzymes, Immobilized* / pharmacokinetics
  • Hydrolysis
  • Models, Biological
  • Models, Molecular
  • Nanotubes, Carbon / chemistry
  • Protein Processing, Post-Translational
  • Solubility
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
  • Trypsin / metabolism
  • Trypsin / pharmacokinetics*
  • Water / metabolism*

Substances

  • Dendrimers
  • Enzymes, Immobilized
  • Nanotubes, Carbon
  • Water
  • Trypsin