Fluorescent mannose-functionalized hyperbranched poly(amido amine)s: synthesis and interaction with E. coli

Wen Yang; Cai-Yuan Pan; Ming-Deng Luo; Hong-Bin Zhang

doi:10.1021/bm100307d

Fluorescent mannose-functionalized hyperbranched poly(amido amine)s: synthesis and interaction with E. coli

Biomacromolecules. 2010 Jul 12;11(7):1840-6. doi: 10.1021/bm100307d.

Authors

Wen Yang¹, Cai-Yuan Pan, Ming-Deng Luo, Hong-Bin Zhang

Affiliation

¹ Department of Polymer Science and Engineering, CAS Key Laboratory of Soft Matter Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, People's Republic of China.

PMID: 20527872
DOI: 10.1021/bm100307d

Abstract

A water-soluble, biodegradable and fluorescent hyperbranched poly(amidoamine) with mannose groups on their surface (M-HPAMAM) has been successfully prepared, and the synthetic strategy includes Michael addition polymerization of diacrylamide with 1-(2-aminoethyl)piperazine and, subsequently, surface modification with mannosamine. The photoluminescence of M-HPAMAM was enhanced significantly due to the surface mannose groups. Incubation of E. coli with M-HPAMAMs yielded brightly fluorescent bacteria clusters, but the fluorescent intensity of the aqueous solution lowered. This indicates that the M-HPAMAMs have strong affinity with bacteria due to their polyvalent interactions. Based on the size and the amount of bacteria clusters formed, the bacteria with the concentrations higher than 10(2) cfu/mL can be detected.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Adhesion
Escherichia coli / isolation & purification*
Fluorescence*
Fluorescent Dyes
Limit of Detection
Mannose / chemistry*
Molecular Probe Techniques
Polyamines / chemical synthesis*

Substances

Fluorescent Dyes
Poly(amidoamine)
Polyamines
Mannose