Objective: To study the effect of Liuwei Dihuang Pill on kidney lesion in rats induced by gentamicin and its mechanism.
Methods: Rats were divided into three groups: control group, gentamicin group, Liuwei Dihuang Pill group randomly. Parameters of kidney function and oxidative production in three groups were tested by biochemical analysis. Nuclear Factor-kappaB (NF-kappaB) protein were determined by western blotting.
Results: Parameters of weight, kidney indexes, oxidative production MDA and enzyme activity of NOS in kidney cortex of gentamicin group significantly increased (P < 0.05), and enzyme activity of SOD significantly decreased (P < 0. 05). The protein expression of NF-kappaB in gentamicin group was significantly up-regulated. After treatment with Liuwei Dihuang Pill, the above-mentioned up-regulated parameters were all significantly inhibited.
Conclusion: Liuwei Dihuang Pill can inhibit the expression of NF-kappaB protein, so as to alleviate the renal oxidative lesion, and it may be one of the important mechanisms for treating gentamicin induced kidney lesion.