Abstract
In an attempt to understand the complex regulatory mechanisms underlying sucrose-induced flavonoid biosynthesis, we examined several Arabidopsis mutants with altered anthocyanin accumulation. We determined that disruption of ethylene signaling results in a dramatic increase in sucrose-induced anthocyanin accumulation. Furthermore, we investigated why the ein2-1 (ethylene insensitive) Arabidopsis mutant accumulates higher levels of anthocyanin in response to sucrose than wild-type Arabidopsis. An increased level of PAP1 transcript in the ein2-1 mutant appears to be the main factor responsible for the increased accumulation of anthocyanin in response to sucrose. Therefore, our results indicate that the ethylene signaling pathway plays a negative role in sucrose-induced anthocyanin accumulation. We believe that the explanation for this observation may be related to the initiation of the senescence program in plants.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acyltransferases / genetics
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Acyltransferases / metabolism
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Anthocyanins / metabolism*
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Arabidopsis / drug effects*
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Arabidopsis / enzymology
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Arabidopsis / genetics
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Arabidopsis / metabolism*
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Arabidopsis Proteins / genetics
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Arabidopsis Proteins / metabolism
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Blotting, Northern
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Chlorophyll / metabolism
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Ethylenes / metabolism*
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Gene Expression Regulation, Plant / drug effects
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Mutation / genetics
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Pancreatitis-Associated Proteins
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Signal Transduction / drug effects*
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Silver Nitrate / pharmacology
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Sucrose / pharmacology*
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Transcription Factors / genetics
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Transcription Factors / metabolism
Substances
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Anthocyanins
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Arabidopsis Proteins
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Ethylenes
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PAP1 protein, Arabidopsis
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Pancreatitis-Associated Proteins
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REG3A protein, human
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RNA, Messenger
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Transcription Factors
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Chlorophyll
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Sucrose
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ethylene
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Silver Nitrate
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Acyltransferases
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AT5G13930 protein, Arabidopsis