Reconstruction of the UDP-N-acetylglucosamine biosynthetic pathway in cell-free system

Jiewen Zhou; Lei Huang; Jiazhang Lian; Jiayuan Sheng; Jin Cai; Zhinan Xu

doi:10.1007/s10529-010-0315-8

Reconstruction of the UDP-N-acetylglucosamine biosynthetic pathway in cell-free system

Biotechnol Lett. 2010 Oct;32(10):1481-6. doi: 10.1007/s10529-010-0315-8. Epub 2010 May 22.

Authors

Jiewen Zhou¹, Lei Huang, Jiazhang Lian, Jiayuan Sheng, Jin Cai, Zhinan Xu

Affiliation

¹ Institute of Biological Engineering, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, China.

PMID: 20495944
DOI: 10.1007/s10529-010-0315-8

Abstract

A functional glucokinase (YqgR), N-acetylglucosamine-phosphate mutase (Agm1) and N-acetylglucosamine-1-phosphate uridyltransferase (GlmU) were synthesized simultaneously in an Escherichia coli cell-free system. These three enzymes were coupled to reconstruct a biosynthetic pathway which could produce UDP-N-acetylglucosamine with N-acetylglucosamine as the substrate.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acetylglucosamine / metabolism*
Bacillus subtilis / enzymology
Biosynthetic Pathways*
Escherichia coli / enzymology
Glucokinase / metabolism
In Vitro Techniques
Intramolecular Transferases / metabolism
Saccharomyces cerevisiae / enzymology
Transferases (Other Substituted Phosphate Groups) / metabolism
Uridine Diphosphate N-Acetylglucosamine / biosynthesis*

Substances

Uridine Diphosphate N-Acetylglucosamine
Glucokinase
Transferases (Other Substituted Phosphate Groups)
UDPacetylglucosamine-dolichyl-phosphate acetylglucosamine-1-phosphate transferase
Intramolecular Transferases
Acetylglucosamine