Fluorescent ratiometric MFC probe sensitive to early stages of alpha-synuclein aggregation

J Am Chem Soc. 2010 Jun 16;132(23):7860-1. doi: 10.1021/ja102838n.

Abstract

We introduce a sensor molecule, AS140-MFC, consisting of a covalent adduct of an Ala-to-Cys mutant of alpha-synuclein with the 3-hydroxychromone dual emission dye MFC. We show that the AS140-MFC construct is a multiparametric fluorescent probe suitable for the continuous monitoring of protein aggregation and is sensitive to the early and intermediate stages of alpha-synuclein aggregation, a process associated with Parkinson's disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Fluorescent Dyes / chemistry*
  • Maleimides / chemistry*
  • Models, Molecular
  • Protein Multimerization*
  • Protein Structure, Quaternary
  • Spectrometry, Fluorescence
  • Sulfhydryl Compounds / chemistry*
  • Time Factors
  • alpha-Synuclein / chemistry*
  • alpha-Synuclein / metabolism*

Substances

  • Fluorescent Dyes
  • Maleimides
  • Sulfhydryl Compounds
  • alpha-Synuclein