Influence of the incorporation of (S)-9-(3,4-dihydroxybutyl)adenine on the enzymatic stability and base-pairing properties of oligodeoxynucleotides

K Augustyns; A Van Aerschot; A Van Schepdael; C Urbanke; P Herdewijn

doi:10.1093/nar/19.10.2587

Influence of the incorporation of (S)-9-(3,4-dihydroxybutyl)adenine on the enzymatic stability and base-pairing properties of oligodeoxynucleotides

Nucleic Acids Res. 1991 May 25;19(10):2587-93. doi: 10.1093/nar/19.10.2587.

Authors

K Augustyns¹, A Van Aerschot, A Van Schepdael, C Urbanke, P Herdewijn

Affiliation

¹ Rega Institute for Medical Research, Leuven, Belgium.

Abstract

(S)-9-(3,4-dihydroxybutyl)adenine was used at several positions as nucleoside substitute in the synthesis of dimers and 13-mers. Therefore we used the phosporamidite and the H-phosphonate chemistry. The nuclease susceptibilities and the base-pairing properties of these oligomers have been evaluated.

MeSH terms

Adenine / analogs & derivatives*
Adenine / chemistry
Base Composition
Enzyme Stability
Molecular Structure
Oligodeoxyribonucleotides / chemistry*
Oligodeoxyribonucleotides / metabolism
Polymers / chemical synthesis
Polymers / metabolism
Temperature

Substances

Oligodeoxyribonucleotides
Polymers
9-(3,4-dihydroxybutyl)adenine
Adenine