Identification of the new polymorphisms in the promoter region of the CAST gene in cattle

E Juszczuk-Kubiak; K Flisikowski; K Wicińska; J Połoszynowicz; S Rosochacki

doi:10.1016/j.meatsci.2009.01.001

Identification of the new polymorphisms in the promoter region of the CAST gene in cattle

Meat Sci. 2009 Jun;82(2):278-83. doi: 10.1016/j.meatsci.2009.01.001. Epub 2009 Jan 9.

Authors

E Juszczuk-Kubiak¹, K Flisikowski, K Wicińska, J Połoszynowicz, S Rosochacki

Affiliation

¹ Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzębiec, 05-552 Wólka Kosowska, Poland.

PMID: 20416736
DOI: 10.1016/j.meatsci.2009.01.001

Abstract

Calpastatin (CAST) is a specific inhibitor of the ubiquitous calcium-dependent proteases -μ-calpain and m-calpain. This proteolytic system plays a key role in the tenderization process that occurs during postmortem storage of meat under refrigerated conditions. In the present study using comparative sequencing seven novel polymorphisms located within P3 promoter region for exon 1u of the bovine CAST gene: -357 (C/G), -556 (G/T), -557 (A/G), -580 (G/C), -750 (T/C), and two InDel at position -890 (A/-) and (GTT/-) at position -353/-351 were found. This region directs the expression of type III calpastatin mRNA, encoding the prototypical calpastatin. The genotype frequencies and haplotypes distribution were studied in 191 bulls belonging to six cattle breeds. All genotypes were distributed according to the HWE test and two major combined haplotypes were identified. The frequency of the haplotype1 varied from 0.45 in Aberdeen Angus to 0.82 in Simmental.