Comparison of muscle proteome profiles in pure breeds of Norwegian Landrace and Duroc at three different ages

Kristin Hollung; Harald Grove; Ellen Mosleth Færgestad; Maan Singh Sidhu; Per Berg

doi:10.1016/j.meatsci.2008.10.003

Comparison of muscle proteome profiles in pure breeds of Norwegian Landrace and Duroc at three different ages

Meat Sci. 2009 Mar;81(3):487-92. doi: 10.1016/j.meatsci.2008.10.003. Epub 2008 Oct 10.

Authors

Kristin Hollung¹, Harald Grove¹, Ellen Mosleth Færgestad¹, Maan Singh Sidhu², Per Berg²

Affiliations

¹ Matforsk AS, Nofima Food, Osloveien 1, N-1430 Ås, Norway.
² Animalia - The Norwegian Meat and Poultry Research Centre, P.O. Box 396, Økern, N-0513 Oslo, Norway.

PMID: 20416601
DOI: 10.1016/j.meatsci.2008.10.003

Abstract

We have used proteomics as a tool to unravel the changes in protein composition between two pure pig breeds and three age groups. Forty two female pigs of Norwegian Landrace and Duroc breed slaughtered at 6, 9 and 12 months age were included in the study. Each of the breeds was raised in separate farms and was slaughtered at the same day in a commercial abattoir. A sample from the adductor muscle was collected approximately 45min postmortem. Proteome analyses of the water soluble proteins using 2D electrophoresis showed that of the 1125 analyzed protein spots, 94 and 41 proteins are changed in abundance according to breed and age, respectively. A total of 63 changed proteins were identified by mass spectrometry. The identified proteins were classified as structural proteins, metabolic proteins, stress/defense proteins and other proteins. This demonstrates a difference in metabolism and muscle composition between breeds and age groups and shows that proteomics is a useful tool to uncover the molecular basis for physiological differences in muscles between pig breeds and age groups.