Detection of white spot syndrome virus (WSSV) in the Pacific oyster Crassostrea gigas

J Invertebr Pathol. 2010 Jul;104(3):245-7. doi: 10.1016/j.jip.2010.04.004. Epub 2010 Apr 14.

Abstract

Oysters Crassostrea gigas were placed at water supply canals of three shrimp farms in Guasave, Mexico where WSSV outbreaks occur. Animals were sampled through April-August and September-December to detect WSSV DNA. By using three different PCR protocols, only oysters from a farm undergoing a WSSV outbreak were found WSSV-positive in gills and digestive gland. Two WSSV amplicons were sequenced and they corresponded over 99% to WSSV genome segments. Results showed that oysters can capture WSSV particles suspended in water. Susceptibility of oysters to WSSV infection and their role as a carrier remain to be determined.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carrier State / veterinary
  • Carrier State / virology
  • Crassostrea / virology*
  • DNA Virus Infections / diagnosis
  • DNA Virus Infections / transmission
  • DNA Virus Infections / veterinary*
  • DNA, Viral / genetics
  • Digestive System / virology
  • Gills / virology
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sequence Homology, Nucleic Acid
  • Shellfish / virology*
  • Water Microbiology
  • Water Supply / analysis
  • White spot syndrome virus 1 / isolation & purification*

Substances

  • DNA, Viral