Abstract
Using both sequence- and function-based metagenomic approaches, multiple antibiotic resistance determinants were identified within metagenomic libraries constructed from DNA extracted from bacterial chromosomes, plasmids, or viruses within an activated sludge microbial assemblage. Metagenomic clones and a plasmid that in Escherichia coli expressed resistance to chloramphenicol, ampicillin, or kanamycin were isolated, with many cloned DNA sequences lacking any significant homology to known antibiotic resistance determinants.
MeSH terms
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Ampicillin / pharmacology
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Anti-Bacterial Agents / pharmacology
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Chloramphenicol / pharmacology
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DNA, Bacterial / genetics*
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DNA, Viral / genetics*
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Drug Resistance, Bacterial*
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Escherichia coli / genetics
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Gene Library
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Kanamycin / pharmacology
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Metagenome*
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Microbial Sensitivity Tests
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Molecular Sequence Data
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Plasmids*
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Sequence Analysis, DNA
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Sewage / microbiology*
Substances
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Anti-Bacterial Agents
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DNA, Bacterial
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DNA, Viral
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Sewage
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Kanamycin
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Chloramphenicol
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Ampicillin
Associated data
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GENBANK/GU720994
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GENBANK/GU720995
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GENBANK/GU720996
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GENBANK/GU720997
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GENBANK/GU720998
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GENBANK/GU720999
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GENBANK/GU721000
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GENBANK/GU721001
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GENBANK/GU721002
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GENBANK/GU721003
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GENBANK/GU721004
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GENBANK/GU721005