Abstract
DJ-1 is a neuroprotective gene mutated in recessive Parkinson's disease (PD). In addition to direct protective functions in neurons, DJ-1 regulates neuroinflammatory signaling in primary mouse brain astrocytes. To assess the influence of DJ-1 on innate immunity signaling in vivo, we have generated djr-1 knockout Caenorhabditis elegans. When grown on pathogenic gram-negative bacteria, djr-1 (-/-) worms showed stronger phosphorylation of p38 mitogen-activated protein kinase (PMK-1) and hyper-induction of PMK-1 target genes. Thus, PD-associated DJ-1 contributes to regulation of innate immunity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Caenorhabditis elegans / genetics
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Caenorhabditis elegans / immunology*
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Caenorhabditis elegans / metabolism*
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Caenorhabditis elegans Proteins / genetics
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Caenorhabditis elegans Proteins / metabolism*
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Gene Expression Regulation / physiology
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Gene Knockout Techniques
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Immunity, Innate / genetics*
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Mitogen-Activated Protein Kinases / metabolism
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / metabolism
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Parkinson Disease / genetics
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Parkinson Disease / immunology
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Parkinson Disease / metabolism
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Phosphorylation / physiology
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Signal Transduction / immunology*
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Zebrafish Proteins / genetics
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Zebrafish Proteins / metabolism
Substances
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Caenorhabditis elegans Proteins
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Nerve Tissue Proteins
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Zebrafish Proteins
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park7 protein, zebrafish
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Mitogen-Activated Protein Kinases
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Pmk-1 protein, C elegans