[Analysis of 16S rDNA sequence of Cronobacter spp]

Wei Sheng Yan Jiu. 2010 Jan;39(1):36-9.
[Article in Chinese]

Abstract

Objective: To analyse the sequence of 16S rDNA of twenty-nine Cronobacter spp. strains isolated from food as well as two type strains.

Methods: Using polymerase chain reaction to obtain the sequences of 16S rDNA of all strains. The multiple cluster analysis and phylogenetic analysis of them were studied by BioNumerics software.

Results: All strains, except ATCC51329 and ES 014, were clustered with the Cronobacter spp. type strain ATCC29544. The Cronobacter spp. type strain ATCC51329 and ES 014 belonged to a different branch. From the sequence analysis of 16S rDNA, all these isolates of Cronobacter spp. except ES 014 could be identified accurately by gene analysis.

Conclusion: All Cronobacter spp. strains isolated from food were identified on genetic level. The phylogenetic relationships among related Cronobacter spp. isolates were studied.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cronobacter sakazakii / classification
  • Cronobacter sakazakii / genetics*
  • Cronobacter sakazakii / isolation & purification*
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / analysis*
  • Food Microbiology*
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • RNA, Ribosomal, 16S