Identification and characterization of Th cell epitopes in MrkD adhesin of Klebsiella pneumoniae

Microb Pathog. 2010 Jul-Aug;49(1-2):8-13. doi: 10.1016/j.micpath.2010.03.009. Epub 2010 Mar 31.

Abstract

In this study, we identified the Th epitopes in MrkD of Klebsiella pneumoniae, an excellent vaccine candidate antigen. By using the RANKPEP prediction algorithm, we have identified and characterized three Th epitopes within the MrkD antigen, which can be recognized by CD4+ T cells from BALB/c (H-2(d)) mice. They were M(221-235), M(175-189), and M(264-278). These epitopes have important value for studying the immune response of K. pneumoniae infection and for designing effective vaccine against K. pneumoniae.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adhesins, Bacterial / chemistry*
  • Adhesins, Bacterial / genetics
  • Adhesins, Bacterial / immunology*
  • Algorithms
  • Amino Acid Sequence
  • Animals
  • Cells, Cultured
  • Epitope Mapping
  • Female
  • Fimbriae Proteins / chemistry*
  • Fimbriae Proteins / genetics
  • Fimbriae Proteins / immunology*
  • Humans
  • Klebsiella Infections / immunology*
  • Klebsiella Infections / microbiology
  • Klebsiella pneumoniae / chemistry
  • Klebsiella pneumoniae / genetics
  • Klebsiella pneumoniae / immunology*
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • T-Lymphocytes, Helper-Inducer / immunology*

Substances

  • Adhesins, Bacterial
  • Klebsiella pneumoniae type 3 fimbrial adhesin
  • Fimbriae Proteins