Emergence of ciprofloxacin-nonsusceptible Streptococcus pyogenes isolates from healthy children and pediatric patients in Portugal

Antimicrob Agents Chemother. 2010 Jun;54(6):2677-80. doi: 10.1128/AAC.01536-09. Epub 2010 Mar 29.

Abstract

We describe 66 ciprofloxacin-nonsusceptible Streptococcus pyogenes isolates recovered from colonized and infected children. The ParC S79A substitution was frequent and associated with the emm6/sequence type 382 (emm6/ST382) lineage. The ParC D83G substitution was detected in two isolates (emm5/ST99 and emm28/ST52 lineages). One isolate (emm89/ST101) had no quinolone resistance-determining region codon substitutions or other resistance mechanisms. Five of 66 isolates were levofloxacin resistant. Although fluoroquinolones are not used in children, they may be putative disseminators of fluoroquinolone-nonsusceptible strains in the community.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Substitution
  • Anti-Bacterial Agents / pharmacology*
  • Antigens, Bacterial / genetics
  • Bacterial Outer Membrane Proteins / genetics
  • Bacterial Typing Techniques
  • Carrier Proteins / genetics
  • Child
  • Ciprofloxacin / pharmacology*
  • Drug Resistance, Bacterial / genetics
  • Humans
  • Molecular Epidemiology
  • Norfloxacin / pharmacology
  • Point Mutation
  • Portugal / epidemiology
  • Streptococcal Infections / drug therapy*
  • Streptococcal Infections / epidemiology
  • Streptococcal Infections / microbiology*
  • Streptococcus pyogenes / classification
  • Streptococcus pyogenes / drug effects*
  • Streptococcus pyogenes / genetics
  • Streptococcus pyogenes / isolation & purification*

Substances

  • Anti-Bacterial Agents
  • Antigens, Bacterial
  • Bacterial Outer Membrane Proteins
  • Carrier Proteins
  • streptococcal M protein
  • Ciprofloxacin
  • Norfloxacin