1,2,4-Triazine-based chromogenic reagents for the detection of microtraces of various metals left on human skin

J Forensic Sci. 2010 May;55(3):747-52. doi: 10.1111/j.1556-4029.2010.01350.x. Epub 2010 Mar 15.

Abstract

This article extends the application of 1,2,4-triazine-based chromogenic reagents to the detection of nonferrous metal traces left on contact with canvas and human skin. The possibility of detection of iron traces resulting from contact with objects made of stainless steel was investigated as well. Additionally, the ability of triazines to form chromatic complexes with Zn(2+), Ni(2+), Co(2+), Cu(2+), Cr(3+), and Al(3+) ions was studied spectrophotometrically. Molar absorption coefficients, ranging from 8.8 to 29.9 x 10(3)/M/cm, provide high sensitivity of 1,2,4-triazines toward nonferrous ions, thus, enabling the detection at concentrations as low as a few muM. The method was sensitive enough to detect traces resulting from a 1-min contact with a stainless steel made object, which is commonly considered as a corrosion-resistant material. The amounts of metal ions transferred to the skin after a 2-min contact with objects made of brass, zinc, and copper were sufficient to develop chromatic imprints.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Forensic Medicine
  • Humans
  • Indicators and Reagents
  • Metals / analysis*
  • Skin / chemistry*
  • Spectrophotometry
  • Touch
  • Triazines

Substances

  • Indicators and Reagents
  • Metals
  • Triazines
  • 1,2,4-triazine