We have studied the effect of the frozen storage time of industrially produced partially-baked wheat bread on starch digestibility, as the conventional production process is substantially modified for this type of bread, and alterations in its nutritional characteristics are therefore possible. The objective of the present study was to determine the effects of frozen storage on in vitro starch digestibility of partially-baked bread after final bake, with the Englyst analytical method. The rapidly digestible starch, expressed as percentage of dry matter +/- 95% confidence interval, fell significantly at 63 days, from 51.9 +/- 2.9 to 42.7 +/- 2.9 whereas the slowly digestible starch increased over this period, from 18.8 +/- 5.5 to 29.9 +/- 2.7 (P < 0.05). During the frozen storage, changes occur in the composition and structure of partially-baked bread, modifying the in vitro starch digestibility, with a significant reduction in rapidly digestible starch, which is associated with a lower glycaemic response.