Comparative study of the effect of basal diet formulation, dietary fat and cholesterol levels on the development of aortic atherosclerotic lesions in B6.KOR-Apoeshl mice

J Oleo Sci. 2010;59(4):161-7. doi: 10.5650/jos.59.161.

Abstract

To optimize the adequate atherogenic diet composition for nutritional atherosclerosis studies utilizing B6.KOR-Apoe(shl) mice, we investigated the effect of dietary cholesterol, AIN formula, and dietary fats on the development of atherosclerosis. Cholesterol supplementation (0.15-2%) for 12 weeks resulted in a dose-dependent increase in the development of atherosclerosis. Furthermore, there were no significant differences in the degree of atherosclerosis between B6.KOR-Apoe(shl) mice fed a modified-AIN-76 diet and those fed a modified-AIN-93M diet containing corn oil or soybean oil for 10 and 12 weeks. The present experiment indicates that the adequate dietary level of cholesterol was 0.15%, and that further studies are necessary to determine the optimal level of various types of dietary oils for nutritional atherosclerosis experiments in B6.KOR-Apoe(shl) mice.

MeSH terms

  • Animals
  • Aortic Diseases / blood
  • Aortic Diseases / etiology*
  • Aortic Diseases / pathology
  • Atherosclerosis / blood
  • Atherosclerosis / etiology*
  • Atherosclerosis / pathology
  • Body Weight
  • Cholesterol, Dietary / administration & dosage
  • Cholesterol, Dietary / adverse effects*
  • Diet, Atherogenic*
  • Dietary Fats / administration & dosage
  • Dietary Fats / adverse effects*
  • Disease Models, Animal
  • Genetic Predisposition to Disease
  • Lipids / blood
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Time Factors

Substances

  • Cholesterol, Dietary
  • Dietary Fats
  • Lipids