Abstract
Dengue virus envelope glycoprotein (E-protein) is the main protein associated with immunity induction. To produce a candidate for subunit vaccines and to provide an antigen for diagnostic kits, it was expressed in a novel plant system using deconstructed viral modules. A truncated version of the E-protein was designed to be expressed alone and co-expressed with Dengue virus structural proteins. As well, the critical domain III of E-protein was fused to hepatitis B core antigen (HBcore). The recombinant proteins were produced in Nicotiana benthamiana plants and were reactive with the anti-E antibody. The fusion was reactive with both anti-E and anti-HBcore antibodies.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies, Viral / immunology
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Artificial Gene Fusion
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Biotechnology / methods*
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Gene Expression*
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Hepatitis B Core Antigens / biosynthesis
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Hepatitis B Core Antigens / genetics
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Hepatitis B Core Antigens / immunology
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Nicotiana / genetics*
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Nicotiana / metabolism*
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / genetics
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Sequence Deletion
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Transgenes*
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Viral Envelope Proteins / biosynthesis*
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Viral Envelope Proteins / genetics*
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Viral Envelope Proteins / immunology
Substances
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Antibodies, Viral
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E-glycoprotein, Dengue virus type 2
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Hepatitis B Core Antigens
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Recombinant Proteins
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Viral Envelope Proteins