Pediculosis capitis is a common problem worldwide. The aim of this study was to estimate the prevalence of pediculosis capitis in children of preschool or school age in Greece, as well as to investigate the potential risk factors. The study included children of preschool and school age from all over Greece, including urban, semi-urban, and rural areas of Greece. A questionnaire with both closed and open-type questions, which was constructed by the researcher and filled in by the children's parents, was used. A total of 5,084 questionnaires were distributed, of which 2,792 were adequately filled and subsequently evaluated. Descriptive statistics and logistic regression models were used. The median age of the children was 8 years (range: 3-13 yrs) and 54% of them were girls. Pediculosis was found to be prevalent in 12% of the tested population. The prevalence of the disease seems to be increased after the age of 10 years and the risk is higher in girls. The number of family members was correlated positively with the risk of being infected with pediculosis at least once. The children's gender, their age, and family size were correlated with the risk of infection.