Efflux pumps, OprD porin, AmpC beta-lactamase, and multiresistance in Pseudomonas aeruginosa isolates from cystic fibrosis patients

Antimicrob Agents Chemother. 2010 May;54(5):2219-24. doi: 10.1128/AAC.00816-09. Epub 2010 Mar 1.

Abstract

Expression of ampC, oprD, mexA, mexC, mexE, and mexX was studied in 25 Pseudomonas aeruginosa isolates from cystic fibrosis patients, including 14 isolates of the Liverpool epidemic strain. Overexpressed mexA or ampC and reduced oprD were associated with beta-lactam resistance. A specific combination of mexR, nalC, and nalD mutations occurred in 11 Liverpool strain isolates, including 7 with upregulated mexA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Outer Membrane Proteins / genetics*
  • Bacterial Proteins / genetics*
  • Cystic Fibrosis / complications*
  • Drug Resistance, Bacterial / genetics
  • Drug Resistance, Multiple / genetics*
  • Electrophoresis, Gel, Pulsed-Field
  • Humans
  • Membrane Transport Proteins / genetics*
  • Mutation
  • Porins / genetics*
  • Pseudomonas Infections / drug therapy*
  • Pseudomonas Infections / microbiology
  • Pseudomonas aeruginosa / drug effects*
  • Pseudomonas aeruginosa / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Up-Regulation / physiology
  • beta-Lactamases / genetics*

Substances

  • Bacterial Outer Membrane Proteins
  • Bacterial Proteins
  • Membrane Transport Proteins
  • MexA protein, Pseudomonas aeruginosa
  • MexC protein, Pseudomonas aeruginosa
  • MexXY protein, Pseudomonas aeruginosa
  • Porins
  • OprD protein, Pseudomonas aeruginosa
  • AmpC beta-lactamases
  • beta-Lactamases