Abstract
Exendin-4 (Ex-4), a peptide secreted from the salivary glands of the Gila monster lizard, can increase pancreatic beta-cell growth and insulin secretion by activating glucagon-like peptide-1 receptor. In this study, we expressed a fusion protein consisting of exendin-4 and the human immunoglobulin heavy chain (Ex-4/IgG-Fc) in E. coli and explored its potential therapeutic use for the treatment of insulin-resistant type 2 diabetes. Here, we show that the Ex-4/IgG-Fc fusion protein induces expression of insulin receptor substrate-2 in rat insulinoma INS-1 cells. Our findings therefore suggest that Ex-4/IgG-Fc overexpressed in E. coli could be used as a potential, long-acting glucagon-like peptide-1 mimetic.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Line, Tumor
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Diabetes Mellitus, Type 2 / drug therapy
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Escherichia coli / metabolism
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Exenatide
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Glucagon-Like Peptide 1 / metabolism
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Glucagon-Like Peptide-1 Receptor
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Humans
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Hypoglycemic Agents / metabolism
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Hypoglycemic Agents / pharmacology*
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Immunoglobulin Fc Fragments / genetics
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Immunoglobulin Heavy Chains / genetics
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Insulin Receptor Substrate Proteins / metabolism*
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Insulin Resistance
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Peptides / genetics
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Peptides / metabolism
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Peptides / pharmacology*
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Rats
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Receptors, Glucagon / metabolism
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Recombinant Fusion Proteins / pharmacology
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Venoms / genetics
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Venoms / metabolism
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Venoms / pharmacology*
Substances
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GLP1R protein, human
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Glp1r protein, rat
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Glucagon-Like Peptide-1 Receptor
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Hypoglycemic Agents
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Immunoglobulin Fc Fragments
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Immunoglobulin Heavy Chains
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Insulin Receptor Substrate Proteins
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Peptides
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Receptors, Glucagon
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Recombinant Fusion Proteins
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Venoms
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Glucagon-Like Peptide 1
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Exenatide