Tribuli fructus constituents protect against tacrine-induced cytotoxicity in HepG2 cells

Arch Pharm Res. 2010 Jan;33(1):67-70. doi: 10.1007/s12272-010-2226-6. Epub 2010 Feb 27.

Abstract

A new phenolic amide, tribulusimide D (4-hydroxy-N-[3-(4-hydroxy-3-methoxyphenyl)-1-oxo-2-propen-1-yl]-3-methoxybenzamide) (1), together with a known phenolic amide, terrestriamide ((E)-3-(4-hydroxy-3-methoxyphenyl)-N-[2-(4-hydroxyphenyl)-2-oxoethyl]-prop-2-enamide) (2) and a flavonol glycoside, quercetin-3-O-beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranoside (3) were isolated from the H2O extract of Tribuli Fructus. Compounds 1 and 3 showed significant hepatoprotective activities, with EC50 values of 13.46 +/- 0.2 and 7.06 +/- 0.7 microM, respectively, against tacrine-induced cytotoxicity in HepG2 cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbohydrate Sequence
  • Cell Line
  • Chemical and Drug Induced Liver Injury / pathology
  • Chemical and Drug Induced Liver Injury / prevention & control*
  • Fruit / chemistry
  • Guaiacol / analogs & derivatives*
  • Guaiacol / chemistry
  • Guaiacol / pharmacology
  • Imides / chemistry*
  • Imides / pharmacology*
  • Molecular Sequence Data
  • Nootropic Agents / toxicity*
  • Plant Extracts / chemistry
  • Protective Agents / chemistry*
  • Protective Agents / pharmacology*
  • Spectrometry, Mass, Electrospray Ionization
  • Tacrine / antagonists & inhibitors*
  • Tacrine / toxicity*
  • Tribulus / chemistry*

Substances

  • 4-hydroxy-N-(3-(4-hydroxy-3-methoxyphenyl)-1-oxo-2-propen-1-yl)-3-methoxybenzamide
  • Imides
  • Nootropic Agents
  • Plant Extracts
  • Protective Agents
  • Tacrine
  • Guaiacol