Canine claws are complex epithelial structures resembling the mammalian hair fibre, and human nail plate, in terms of tissue-specific differentiation. They are composed of several distinct epithelial cell lineages undergoing either hard or soft keratinization. The claw plate has three distinct regions: stratum externum, stratum medium (SM) and stratum internum and the underside and tip are cushioned by a soft keratinizing epithelium, the sole. We have examined keratin expression in the canine claw and associated epithelia. Digits from German shepherd dogs were decalcified, processed and sectioned by sledge microtome. Sections were stained with haematoxylin and eosin or treated with specific antibodies to various keratins (immunohistochemistry). Proteins were extracted from claw components and analysed by SDS-PAGE and Western blotting. The keratinized canine claw plate expressed hair-specific keratins (type I, K25-K38 and type II, K71-K86) but only the inner region of the SM contained K6- and K16-positive tubules, soft epithelia running through the hard keratinized claw plate. The soft keratinaceous sole epithelium expressed keratins K5, K6, K14, K16 and K17 and contained cells with abundant envelopes. The canine claw had two slippage zones, the inner claw bed, between the claw plate and ungula process, which expressed K17 and the region between the inner and outer claw sheath, equivalent to the hair follicle companion layer, which expressed K6, K77, K16 and K17. In conclusion, several different cell types have been defined in the canine claw presenting a complex mechanism of cellular differentiation.