A microfluidic approach for high-throughput droplet interface bilayer (DIB) formation

Chem Commun (Camb). 2010 Mar 14;46(10):1620-2. doi: 10.1039/b924897h. Epub 2010 Jan 25.

Abstract

We present a simple, automated method for high-throughput formation of droplet interface bilayers (DIBs) in a microfluidic device. We can form complex DIB networks that are able to fill predefined three dimensional architectures. Moreover, we demonstrate the flexibility of the system by using a variety of lipids including 1,2-diphytanoyl-sn-glycero-3-phosphocholine (DPhPC) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Fluorescein / chemistry
  • Lipid Bilayers / chemistry*
  • Microfluidic Analytical Techniques / instrumentation*
  • Microfluidic Analytical Techniques / methods*
  • Oils / chemistry
  • Phosphatidylcholines / chemistry
  • Water / chemistry

Substances

  • Lipid Bilayers
  • Oils
  • Phosphatidylcholines
  • Water
  • 1,2-diphytanoylphosphatidylcholine
  • 1,2-oleoylphosphatidylcholine
  • Fluorescein