Development of a loop-mediated isothermal amplification assay for the detection of Mycobacterium bovis

Jun Zhang; Gui-Hong Zhang; Lin Yang; Ren Huang; Yu Zhang; Kun Jia; Wen Yuan; Shou-Jun Li

doi:10.1016/j.tvjl.2010.01.001

Development of a loop-mediated isothermal amplification assay for the detection of Mycobacterium bovis

Vet J. 2011 Mar;187(3):393-6. doi: 10.1016/j.tvjl.2010.01.001. Epub 2010 Feb 7.

Authors

Jun Zhang¹, Gui-Hong Zhang, Lin Yang, Ren Huang, Yu Zhang, Kun Jia, Wen Yuan, Shou-Jun Li

Affiliation

¹ College of Veterinary Medicine, South China Agricultural University, 483 Wushan Street, Guangzhou 510642, People's Republic of China.

PMID: 20138787
DOI: 10.1016/j.tvjl.2010.01.001

Abstract

Bovine tuberculosis caused by Mycobacterium bovis is an important zoonosis. In this study, a simple, rapid method for detecting this organism was developed based on loop-mediated isothermal amplification of the mpt83 gene. The technique will be of value in the clinical and field-based diagnosis of M. bovis and can differentiate it from other bacteria such as Corynebacterium diphtheriae, Streptococcus pneumoniae, β-haemolytic streptococci, Pseudomonas aeruginosa, Yersinia pseudotuberculosis, Staphylococcus aureus, and Klebsiella pneumoniae. The limit of detection was 10 copies/μL and the results were corroborated by PCR. The method was highly specific and more sensitive than PCR.

Publication types

Research Support, Non-U.S. Gov't
Validation Study

MeSH terms

Animals
Cattle
Gene Amplification
Mycobacterium bovis / genetics
Mycobacterium bovis / isolation & purification*
Nucleic Acid Amplification Techniques / methods
Nucleic Acid Amplification Techniques / standards
Nucleic Acid Amplification Techniques / veterinary*
Polymerase Chain Reaction / veterinary
Reproducibility of Results
Sensitivity and Specificity
Sputum / microbiology
Tuberculosis, Bovine / diagnosis*