A multiresidue, multiclass semiquantitative screening analysis of 39 drug residues covering 8 drug classes, including aminoglycosides in veal muscle, based on a single multiresidue extraction routine and using high-performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS), is presented. Sample preparation involves extraction of a 5 g diced tissue sample with 10 mL of acetonitrile/ water (86:14), incubated at 60 degrees C for 1 h, and then cooled for 10 min in ice. Formic acid is added to the suspension, then mixed, and centrifuged. The supernatant is retained, and the pellet is extracted with 10 mL of water for aminoglycosides and again centrifuged. Approximately 9.5 mL of each of the supernatants from both extracts is combined and diluted with water to 25 mL. The final solution is then defatted with 20 mL of hexane prior to analysis. Liquid chromatography for the aminoglycosides is carried out with ZIC-HILIC and for the remainder of the compounds with an Atlantis dC18 minicolumn. LC-ESI-MS/MS in positive and negative ionization modes (three injections total) is carried out, and two ion transitions per analyte are monitored. The method provides semiquantitative analysis to identify incurred positive drug classes in a rapid and cost-effective manner. Of particular interest is the detection of numerous compounds in the low nanograms per gram concentration range, which are not typically detected using receptor-based screening methods. All identified drugs were confirmed using internationally recognized regulatory methods, with no apparent false positives.