Ciprofloxacin-resistant, CTX-M-15-producing Escherichia coli ST131 clone in extraintestinal infections in Italy

Clin Microbiol Infect. 2010 Oct;16(10):1555-8. doi: 10.1111/j.1469-0691.2010.03162.x.

Abstract

Quinolone and β-lactam resistance mechanisms and clonal relationships were characterized among Escherichia coli isolates resistant to ciprofloxacin and extended-spectrum cephalosporins associated with human extra-intestinal infections in Rome. The E. coli. ST131 clone was found to be prevalent. This clone invariably carried a specific pattern of substitutions in the topoisomerase genes and all isolates but one produced CTX-M-15. One ST131 isolate produced SHV-12. The new ST131 variant described here is of particular concern because it combines fluoroquinolone resistance and chromosomally encoded CTX-M-15.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Substitution / genetics
  • Anti-Bacterial Agents / pharmacology
  • Bacterial Typing Techniques
  • Ciprofloxacin / pharmacology*
  • Cluster Analysis
  • DNA Topoisomerase IV / genetics
  • Drug Resistance, Multiple, Bacterial*
  • Escherichia coli / classification
  • Escherichia coli / drug effects*
  • Escherichia coli / enzymology*
  • Escherichia coli / isolation & purification
  • Escherichia coli Infections / epidemiology*
  • Escherichia coli Infections / microbiology
  • Humans
  • Microbial Sensitivity Tests
  • Molecular Epidemiology
  • Molecular Typing
  • Mutation, Missense
  • Rome / epidemiology
  • beta-Lactamases / biosynthesis*

Substances

  • Anti-Bacterial Agents
  • Ciprofloxacin
  • beta-lactamase CTX-M-15
  • beta-Lactamases
  • DNA Topoisomerase IV