Tn5090-like class 1 integron carrying bla(VIM-2) in a Pseudomonas putida strain from Portugal

Clin Microbiol Infect. 2010 Oct;16(10):1558-61. doi: 10.1111/j.1469-0691.2010.03165.x.

Abstract

Three Pseudomonas putida strains containing bla(VIM-2) were isolated from an inanimate surface of a female ward sanitary facility in the Hospital Infante D. Pedro, Aveiro. A novel class 1 integron was found in strain Pp2 (aacA4/bla(VIM-2)/aac6'-IIc disrupted by an insertion sequence IS1382), and strain Pp1 was found to carry a class 1 integron (aacA7/bla(VIM-2)/aacC1/aacA4), which is described for the first time in this species. Strain PF1 carries a class 1 integron associated with a Tn5090-like transposon, constituting the first finding of this type of arrangement in a strain from Portugal. This association highlights further dissemination of bla(VIM-2) in environmental hospital isolates.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Transposable Elements
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Environmental Microbiology*
  • Female
  • Gene Order
  • Hospitals
  • Humans
  • Integrons*
  • Molecular Sequence Data
  • Portugal
  • Pseudomonas putida / drug effects*
  • Pseudomonas putida / genetics*
  • Pseudomonas putida / isolation & purification
  • Sequence Analysis, DNA
  • beta-Lactamases / genetics*

Substances

  • DNA Transposable Elements
  • DNA, Bacterial
  • beta-lactamase bla(vim-2)
  • beta-Lactamases

Associated data

  • GENBANK/FJ715942