Effect of MTAD on the differentiation of osteoblast-like cells

Yoshiyuki Yasuda; Yuki Tatematsu; Shinsuke Fujii; Hidefumi Maeda; Akifumi Akamine; Mahmoud Torabinejad; Takashi Saito

doi:10.1016/j.joen.2009.11.002

Effect of MTAD on the differentiation of osteoblast-like cells

J Endod. 2010 Feb;36(2):260-3. doi: 10.1016/j.joen.2009.11.002.

Authors

Yoshiyuki Yasuda¹, Yuki Tatematsu, Shinsuke Fujii, Hidefumi Maeda, Akifumi Akamine, Mahmoud Torabinejad, Takashi Saito

Affiliation

¹ Division of Clinical Cariology and Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido, Japan. yasuda@hoku-iryo-u.ac.jp

PMID: 20113785
DOI: 10.1016/j.joen.2009.11.002

Abstract

Introduction: The aim of the present study was to investigate the effect of MTAD on the differentiation of osteoblast-like cells.

Methods: The cell viability assay was performed to evaluate the cytotoxicity of MTAD on MC3T3-E1 and periodontal ligament cells at the various concentrations. The bone sialoprotein (BSP) gene expression was also examined by real-time polymerase chain reaction.

Results: MTAD exhibited a lower cytotoxicity compared with other intracanal irrigants and medication. The MC3T3-E1 cells treated with H2O2 showed a decrease in the alkaline phosphatase (ALP) activity by 40% on day 14 compared with the control group at the concentration of 50 microg/mlL. No significant difference in the ALP activity was observed between MTAD and control group. Furthermore, MTAD and Ca(OH)2 paste did not change in BSP gene expression in MC3T3-E1 cells on day 21.

Conclusions: These results suggested that MTAD is a less cytotoxic irrigant and does not affect differentiation into osteoblasts compared with other intracanal irrigants, such as H2O2, NaOCl, ethylenediaminetetraacetic acid, and chlorhexidine.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Alkaline Phosphatase / drug effects
Alkaline Phosphatase / metabolism
Analysis of Variance
Animals
Cell Differentiation / drug effects*
Cell Line
Cell Survival / drug effects
Citric Acid / toxicity*
Doxycycline / toxicity*
Drug Combinations
Fibroblasts / cytology
Fibroblasts / drug effects
Fibroblasts / metabolism
Humans
Integrin-Binding Sialoprotein
Mice
Osteoblasts / cytology
Osteoblasts / drug effects*
Osteoblasts / metabolism
Periodontal Ligament / cytology
Periodontal Ligament / drug effects*
Periodontal Ligament / metabolism
Polysorbates / toxicity*
Root Canal Irrigants / toxicity*
Sialoglycoproteins / drug effects
Sialoglycoproteins / metabolism
Statistics, Nonparametric

Substances

Drug Combinations
IBSP protein, human
Ibsp protein, mouse
Integrin-Binding Sialoprotein
MTAD (intracanal irrigant)
Polysorbates
Root Canal Irrigants
Sialoglycoproteins
Citric Acid
Alkaline Phosphatase
Doxycycline