AChE biosensor based on zinc oxide sol-gel for the detection of pesticides

Ravi Sinha; Mallikarjunarao Ganesana; Silvana Andreescu; Lia Stanciu

doi:10.1016/j.aca.2009.12.020

AChE biosensor based on zinc oxide sol-gel for the detection of pesticides

Anal Chim Acta. 2010 Feb 28;661(2):195-9. doi: 10.1016/j.aca.2009.12.020. Epub 2009 Dec 22.

Authors

Ravi Sinha¹, Mallikarjunarao Ganesana, Silvana Andreescu, Lia Stanciu

Affiliation

¹ School of Materials Engineering, Purdue University, 701 West Stadium Avenue, West Lafayette, IN 47907, USA.

PMID: 20113735
DOI: 10.1016/j.aca.2009.12.020

Abstract

Zinc oxide has been used as a matrix for immobilization of acetylcholinesterase (AChE) and detection of the pesticide paraoxon. The immobilized enzyme retained its enzymatic activity up to three months when stored in phosphate buffered saline (pH 7.4) at 4 degrees C. An amperometric biosensor for the detection of paraoxon was designed. The biosensor detected paraoxon in the range 0.035-1.38 ppm and can be used to detect other AChE inhibiting organophosphate pesticides.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Acetylcholinesterase / chemistry*
Acetylcholinesterase / metabolism*
Electrochemistry
Enzymes, Immobilized / chemistry*
Enzymes, Immobilized / metabolism*
Gels
Paraoxon / analysis
Pesticides / analysis*
Surface Plasmon Resonance / methods*
Zinc Oxide / chemistry*

Substances

Enzymes, Immobilized
Gels
Pesticides
Acetylcholinesterase
Paraoxon
Zinc Oxide