Spatial and temporal expression of spermadhesin genes in reproductive tracts of male and female pigs and ejaculated sperm

Abstract

Spermadhesins, a novel protein family identified in the reproductive tract of ungulates, have important roles in reproduction. In this study, the expression of pig (Sus domesticus) spermadhesion genes in seminal vesicles, prostate, and bulbourethral glands from birth to sexual maturity and the spatial expression in adult male and female genital tracts and ejaculated sperm of Meishan pigs were evaluated by reverse transcription-polymerase chain reaction (RT-PCR). In general, all spermadhesin genes increased from Days 1 to 150 in the seminal vesicle and bulbourethral gland. However, their expression in the prostate was variable; it increased from Days 1 to 60 and then declined until Day 150. In adult boars, all genes had a very high level of expression in the seminal vesicle and somewhat lower (but still relatively high) in the prostate, caput and caudal epididymides, and bulbourethral gland. Expression of AQN1 and AQN3 was not detectable in the corpus epididymis. In the testis, AQN3 gene expression was not detectable, and gene expressions were weak for AQN1, PSP-I, and PSP-II, but strong for AWN. In female pigs, most spermadhesins had low expression in the cervix, uterine horn, oviduct, and ovary. Expression of AQN1 and AQN3 was very weak in the cervix and uterine horn. Signals for AQN1 in oviduct and ovary and AQN3 in ovary were not detectable, whereas AWN had high expression in the cervix and uterine horn. In ejaculated sperm, a strong mRNA signal of spermadhesins was detected. We concluded that transcripts of spermadhesins were not only distributed extensively in male and female reproductive tissues but also in ejaculated sperm. Furthermore, their dynamic changes of expression paralleled reproductive development. Seminal vesicles were the main source of spermadhesins; when the boar reached puberty, expression of spermadhesins reached very high levels.