Proteomic analysis of porcine saliva

Vet J. 2011 Mar;187(3):356-62. doi: 10.1016/j.tvjl.2009.12.020. Epub 2010 Jan 22.

Abstract

Saliva contains a number of proteins that may be useful as biomarkers of health and disease and can be easily obtained from large numbers of animals in a non-invasive, stress-free way. The objective of this study was to explore the protein composition of porcine saliva from 10 specific pathogen free pigs using first one-dimensional SDS-PAGE and then two-dimensional electrophoresis and mass spectrometry. A reference proteome pattern for porcine saliva was established with the identification of 13 different, mainly saliva-specific, proteins. These reference data will facilitate the investigation of salivary proteins potentially altered in disease and could serve as novel diagnostic biomarkers.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biomarkers / analysis
  • Electrophoresis, Gel, Two-Dimensional
  • Electrophoresis, Polyacrylamide Gel / veterinary
  • Male
  • Mass Spectrometry / veterinary
  • Proteome / chemistry*
  • Proteome / isolation & purification
  • Saliva / chemistry*
  • Salivary Proteins and Peptides / chemistry*
  • Salivary Proteins and Peptides / isolation & purification
  • Specific Pathogen-Free Organisms
  • Swine
  • Swine Diseases / diagnosis

Substances

  • Biomarkers
  • Proteome
  • Salivary Proteins and Peptides