Cyclic voltammetry (CV) coupled with UV-vis and fluorescence spectroscopy were used to probe the interaction of potential anticancer drug, 4-nitrophenylferrocene (NFC) with DNA. The electrostatic interaction of the positively charged NFC with the anionic phosphate of DNA was evidenced by the findings like negative formal potential shift in CV, ionic strength effect, smaller bathochromic shift in UV-vis spectroscopy, incomplete quenching in the emission spectra and decrease in viscosity. The diffusion coefficients of the free and DNA bound forms of the drug were evaluated from Randles-Sevcik equation. The binding parameters like binding constant, ratio of binding constants (K(red)/K(ox)), binding site size and binding free energy were determined from voltammetric data. The binding constant was also determined from UV-vis and fluorescence spectroscopy with a value quite close to that obtained from CV.
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