16S rRNA gene sequencing in routine identification of anaerobic bacteria isolated from blood cultures

J Clin Microbiol. 2010 Mar;48(3):946-8. doi: 10.1128/JCM.02075-09. Epub 2010 Jan 13.

Abstract

A comparison between conventional identification and 16S rRNA gene sequencing of anaerobic bacteria isolated from blood cultures in a routine setting was performed (n = 127). With sequencing, 89% were identified to the species level, versus 52% with conventional identification. The times for identification were 1.5 days and 2.8 days, respectively.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Bacteria, Anaerobic / classification
  • Bacteria, Anaerobic / genetics
  • Bacteria, Anaerobic / isolation & purification*
  • Bacterial Infections / diagnosis
  • Bacteriological Techniques / methods*
  • Blood / microbiology*
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics*
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics*
  • Humans
  • RNA, Ribosomal, 16S / genetics*
  • Sensitivity and Specificity
  • Sequence Analysis, DNA / methods*
  • Time Factors

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • RNA, Ribosomal, 16S