Objective: To compare the agronomic characters, HPLC fingerprints, the content of main component and amino acid between erective and creepy Ophiopogon japonicus of Sichuan.
Method: Agronomic characters were measured by conventional methods; HPLC was applied on a C18 column with CH3OH-CH3CN-2% CH2COOH solution by gradient elution, quercetin was used as the internal standard reference, the contents of total saponins, flavone and polysaccharide were determined by UV spectrophotometry and amino acid was determined by automatic amino acid analyzer.
Result: There were extremely significant differences in the most agronomic characters between erective and creepy O. japonicus of Sichuan. The yield per plant was closely relevant to the roots number and the fresh weight of aerial part. The differences were not significant both in 69 common peaks and 23 uncommon peaks in HLPC fingerprints and the content of main component between erective and creepy O. japonicus.
Conclusion: There is no obvious difference in chemistry component between the two types of O. japonicus. The yield per plant was closely relevant to the roots number and the fresh weight of aerial part. In cultivation it is appropriate to choose the creepy O. japonicus.