Individuals with impaired lipid and glucose metabolism are at increased risk for postprandial oxidative stress. Acute exercise can attenuate the rise in both blood triglyceride (TAG) and glucose, and increase antioxidant enzyme activity after food intake, which may decrease the oxidative stress response. This study investigated the effect of acute exercise on blood TAG and oxidative stress biomarkers in prediabetic women. Sixteen prediabetic women (30 +/- 3 years of age; fasting blood glucose, 107 +/- 3 mg x dL(-1); body mass index, 32 +/- 2 kg x m(-2)) consumed a high-fat meal with and without a session of aerobic exercise 15 minutes preceding the meal (45-minute duration, 65% heart rate reserve), in a random order cross-over design. Blood samples were collected premeal (fasted) and at 1, 2, 4, and 6 hours postmeal and assayed for Trolox equivalent antioxidant capacity (TEAC), xanthine oxidase (XO) activity, hydrogen peroxide (H2O2), malondialdehyde (MDA), TAG, and glucose. No interaction or condition main effects were noted (P > 0.05). However, time main effects were noted for XO, H2O2, MDA, and TAG (P < 0.0001), with values higher from 1 to 6 hours postmeal compared with premeal, and for TEAC (P = 0.05), with values lower at 4 hours postmeal. Glucose remained relatively unchanged (P > 0.05). Acute exercise, performed at the intensity and duration of the present study, does not influence postprandial TAG and oxidative stress in obese prediabetic women. Such individuals may need a greater volume of exercise for measurable effects.