Mechanism of inactivation of murine norovirus-1 by high pressure processing

Qingjuan Tang; Dan Li; Jie Xu; Jingfeng Wang; Yuran Zhao; Zhaojie Li; Changhu Xue

doi:10.1016/j.ijfoodmicro.2009.10.033

Mechanism of inactivation of murine norovirus-1 by high pressure processing

Int J Food Microbiol. 2010 Feb 28;137(2-3):186-9. doi: 10.1016/j.ijfoodmicro.2009.10.033. Epub 2009 Nov 5.

Authors

Qingjuan Tang¹, Dan Li, Jie Xu, Jingfeng Wang, Yuran Zhao, Zhaojie Li, Changhu Xue

Affiliation

¹ College of Food Science and Engineering, Ocean University of China, No 5, Yushan Road, Qingdao, Shandong Province 266003, PR China.

PMID: 20034691
DOI: 10.1016/j.ijfoodmicro.2009.10.033

Abstract

Murine norovirus-1 (MNV) is currently the most suitable surrogate for human norovirus. The mechanism of MNV-1 inactivation by high pressure processing (HPP) was investigated. HPP-treated MNV could not bind to its target receptor and therefore could not initiate infection of mouse RAW cells. The integrity of the capsid was not affect by HPP. Partial motif changes of the viral capsid caused by HPP were accessed by induced sensitivity to proteinase K.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Line
Endopeptidase K / metabolism
Hydrostatic Pressure*
Macrophages / virology
Mice
Microbial Viability*
Norovirus / physiology*
Viral Proteins / metabolism
Virus Inactivation*

Substances

Viral Proteins
Endopeptidase K