Objective: To identify two marine fungi and evaluate the inhibitory effects of their crude extracts on Tobacco mosaic virus and two tumor cell lines.
Methods: Crude extracts was obtained by extracting with MeOH and evaporated in vacuo. The extracts was water-soluble fraction which was dissolved in water, and the other fraction was water insoluble. The fungi were identified by morphology and Internal Transcribed Spcer (ITS) rDNA molecular methods. The inhibitory effect on Tobacco mosaic virus was evaluated by indirect enzyme linked immunosorbent assay, and the anti-tumor activity was tested by methyl thiazolyl tetrazolium method.
Results: The fungi were identified as Penicillium oxalicum and Neosartorya fischeri. There crude extracts inhibited Tobacco Mosaic Virus and two tumor cell lines. The active fraction named 0312F1 inhibited Tobacco Mosaic Virus and tumor cell lines and was water-soluble. The fraction named 1008F1 inhibited Tobacco Mosaic Virus and was insoluble in water, whereas the fraction inhibited tumor cell lines was water-soluble.
Conclusion: The active fraction named 0312F1 inhibited Tobacco Mosaic Virus was different from that named 1008F1 inhibited Tobacco Mosaic Virus. The active fraction named 0312F1 inhibited tumor cell lines was the same as that named 1008F1. Furthermore, the inhibitory activity of water-soluble fraction named 0312F1 against BEL-7404 cell line was much higher than that against SGC-7901 cell lines, whereas the inhibitory activity of active fraction named 1008F1 against SGC-7901 cell line was much higher.