This paper studies the feasibility of spatio-temporal gait analysis based upon digital textile sensors. Digitized legs and feet patterns of healthy subjects and their relations with spatio-temporal gait parameters were analyzed. In the first experiment, spatio-temporal gait parameters were determined during over ground walking. In the second experiment, predicted running, backward walking, walking up stairs and walking down stairs parameters were determined. From the results of the experiments, it is concluded that, for healthy subjects, the duration of subsequent stride cycles and left/right steps, the estimations of step length, cadence, walking speed, central of pressure and central of mass trajectory, can be obtained by analyzing the digital signals from the textile sensors on pants and socks. These parameters are easily displayed in several different graphs allowing the user to view the parameters during gait. Finally, the digital data are easily to analyze the feature of activity recognition.