A crucial step in assisted reproduction technology: human embryo selection using metabolomic evaluation

Fertil Steril. 2010 Jul;94(2):772-4. doi: 10.1016/j.fertnstert.2009.10.013. Epub 2009 Dec 3.

Abstract

We present a new methodology to predict embryo viability in assisted reproductive technology (ART) treatments by determining the relative amino acid concentrations in human embryo culture medium on day 3, using high-performance liquid chromatography with mass spectroscopy analysis without derivatization. The model was performed with soft independent modeling of class analogy for the samples from nonpregnancy and pregnancy cases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acids / metabolism
  • Blastocyst / metabolism
  • Chromatography, Liquid
  • Culture Media, Conditioned / metabolism*
  • Female
  • Humans
  • Mass Spectrometry
  • Metabolome*
  • Models, Biological*
  • Pregnancy
  • Preimplantation Diagnosis / methods*
  • Reproductive Techniques, Assisted*

Substances

  • Amino Acids
  • Culture Media, Conditioned