Cadmium is a metal with no biological function in superior organisms and it is very toxic even at very low concentrations. Thus the objective of this study was to verify some toxicological parameters in silver catfish (Rhamdia quelen) exposed to cadmium. In this study, silver catfish was exposed to 0 (control), 0.236, and 0.414 mg L(-1) cadmium for 7 and 14 days, followed by the same periods of recovery. The effects of cadmium on acetylcholinesterase (AChE) activity and metal accumulation in brain and muscle were verified. Thiobarbituric acid-reactive substance (TBARS) formation was evaluated in brain. An increase in TBARS levels was verified after exposure and recovery periods and AChE activity in brain was reduced after 14 days of exposure. These parameters did not return to control values after the recovery period. In muscle AChE was altered during both exposure periods. Alterations in AChE activity may be a good indicator of cadmium contamination in R. quelen.