Abstract
The study of dendritic cell involvement in complex phenomena that rely on multi-cellular interactions, such as immune homeostasis, stimulation, and tolerization, called for the investigation of dendritic cell functions within physiological context. To this end we have developed a conditional cell ablation strategy that is based on dendritic cell-restricted expression of a Diphtheria Toxin receptor (DTR) using the CD11c/Itgax promoter. Here, we provide basic protocols that describe the use of this prototypic dendritic cell ablation model and highlight pitfalls and strengths of the approach.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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CD11c Antigen / genetics*
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CD11c Antigen / immunology
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Dendritic Cells / cytology
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Dendritic Cells / metabolism
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Dendritic Cells / physiology*
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Flow Cytometry
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Heparin-binding EGF-like Growth Factor
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Intercellular Signaling Peptides and Proteins / chemistry
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Intercellular Signaling Peptides and Proteins / genetics*
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Intercellular Signaling Peptides and Proteins / immunology
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Mice
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Mice, Transgenic
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Models, Biological
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Phagocytes / immunology
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Phagocytes / metabolism
Substances
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CD11c Antigen
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Hbegf protein, mouse
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Heparin-binding EGF-like Growth Factor
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Intercellular Signaling Peptides and Proteins