Structural determination of three neutral oligosaccharides in bovine (Holstein-Friesian) colostrum, including the novel trisaccharide; GalNAc alpha 1-3Gal beta 1-4Glc

T Urashima; T Saito; K Ohmisya; K Shimazaki

doi:10.1016/0304-4165(91)90207-w

Structural determination of three neutral oligosaccharides in bovine (Holstein-Friesian) colostrum, including the novel trisaccharide; GalNAc alpha 1-3Gal beta 1-4Glc

Biochim Biophys Acta. 1991 Jan 23;1073(1):225-9. doi: 10.1016/0304-4165(91)90207-w.

Authors

T Urashima¹, T Saito, K Ohmisya, K Shimazaki

Affiliation

¹ Department of Bioresource Chemistry, Obihiro University of Agriculture and Veterinary Medicine, Japan.

PMID: 1991141
DOI: 10.1016/0304-4165(91)90207-w

Abstract

Two trisaccharides, and a pentasaccharide were obtained from bovine colostrum. Their chemical structures were determined by using methylation and 13C-NMR analyses as follows: GalNac alpha 1-3Gal beta 1-4Glc, Gal alpha-1-3Gal beta 1-4Glc, GaL beta 1-3[Gal beta 1-4GlcNAc beta 1-6]Gal beta 1-4Glc. GalNAc alpha 1-3Gal beta 1-4Glc, which was identified in this study, is a novel oligosaccharide from natural sources. Gal alpha 1-3Gal beta 1-4Glc and Gal beta 1-3[Gal beta 1-4GlcNAc beta 1-6]Gal beta 1-4Glc (lacto-N-novopentaose) have been already found in ovine colostrum, and in horse colostrum and marsupial milk, respectively.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acetylgalactosamine / chemistry
Animals
Carbohydrate Sequence
Cattle
Chromatography, Paper
Colostrum / chemistry*
Magnetic Resonance Spectroscopy
Oligosaccharides / chemistry*
Oxidation-Reduction

Substances

Oligosaccharides
Acetylgalactosamine