A new generation of cross-linkers for selective detection by MALDI MS

Proteomics. 2009 Dec;9(23):5384-8. doi: 10.1002/pmic.200900562.

Abstract

We designed a new cross-linker bearing a CHCA moiety. The use of the CHCA-tagged cross-linker JMV 3378 in conjunction with a neutral MALDI matrix alpha-cyano-4-hydroxycinnamic methyl ester enabled specific signal enhancement in MALDI-TOF MS of cross-link containing peptides. Discrimination between modified and non-modified peptides can be achieved by comparison of two spectra, one using CHCA and the other using the alpha-cyano-4-hydroxycinnamic methyl ester matrix. The methodology was validated using cytochrome c and apo-myoglobine as model proteins.

Publication types

  • Validation Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Apoproteins / analysis
  • Cross-Linking Reagents / chemical synthesis
  • Cross-Linking Reagents / chemistry*
  • Cytochromes c / analysis
  • Horses
  • Molecular Sequence Data
  • Molecular Structure
  • Myoglobin / analysis
  • Peptides / analysis
  • Protein Conformation
  • Proteins / analysis*
  • Sensitivity and Specificity
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

  • Apoproteins
  • Cross-Linking Reagents
  • Myoglobin
  • Peptides
  • Proteins
  • apomyoglobin
  • Cytochromes c