Compounds 4-(4-morpholinophenyl)-6-phenylpyrimidin-2-amine 20, 4-(4-methoxyphenyl)-6-(4-morpholinophenyl)pyrimidin-2-amine 23, 4-(4-bromophenyl)-6-(4-morpholinophenyl)pyrimidin-2-amine 25, 4-(3-chlorophenyl)-6-(4-morpholinophenyl) pyrimidin-2-amine 27, and 4-(3-fluorophenyl)-6-(4-morpholinophenyl)pyrimidin-2-amine 28 exerted excellent antibacterial activity against V. cholerae. Compounds 4-(4-chlorophenyl)-6-(4-morpholinophenyl)pyrimidin-2-amine 22, 25, 4-(4-morpholinophenyl)-6-(3-nitrophenyl)pyrimidin-2-amine 26, and 28, which contained electron-withdrawing chloro, bromo, nitro, and fluoro functional groups, respectively, at the para/meta position of the phenyl ring attached to the pyrimidine ring promoted much activity against S. aureus. Compounds 4-(4-fluorophenyl)-6-(4-morpholinophenyl)pyrimidin-2-amine 24 and 25 (against beta-hemolytic Streptococcus) and compound 28 (against S. felxneri) showed pronounced activity. Compounds 26 and 28 (against K. pneumoniae) and compounds 24, 25, and 28 (against P. aeruginosa) exerted strong antibacterial activity. Compounds 22 and 25 promoted much antifungal activity against A. flavus, while compounds 24 and 25 registered maximum activity against Mucor. Compounds 23 (against C. albicans) and 27 and 28 (against Rhizopus) promoted good activity.