Objective: Our research objective is to obtain the active substances from culture liquid of Hypoxylon perforatum with inhibitory effect on Sphaeropsis sapinea growth and germination.
Methods: Water and ester were used for extracting active substances from culture liquid of Hypoxylon perforatum, either by extracting directly from cultural liquid, or extracting under ultrasonic. Growth-inhibiting rate and germination-inhibiting rate were used as the index of antifungal activity.
Results: Five extracts from culture liquid of Hypoxylon perforatum showed antifungal activities to Sphaeropsis sapinea growth and germination.
Conclusion: Ethyl acetate-extract from culture liquid of Hypoxylon perforatum has a highly antifungal activity to Sphaeropsis sapinea, high stability in the natural environment. So it has a highly development of the value and application prospect. The mainly inhibiting active substance is methyl p-methoxy cinnamate, which as medicine intermediate was used in cosmetic to be UV-protection absorbefacient. Diisobutyl phthalate and Dibutyl phthalate are all the common plastic-enhancer for PolyvinylChloride (PVC). It is very important in medicinal industry and chemistry industry to discovering the natural presence of the three compounds.